Main Information

Target

ATG4b

Host Species

Rabbit

Reactivity

Human, Mouse, Rat

Applications

IHC, IF

MW

44kD (Observed)

Conjugate/Modification

Unmodified

Detailed Information

Recommended Dilution Ratio

IHC 1:50-300; IF 1:50-200

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Specificity

The antibody detects endogenous ATG4b protein

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

Concentration

1 mg/ml

MW(Observed)

44kD

Modification

Unmodified

Clonality

Polyclonal

Isotype

IgG

Antigen&Target Information

Immunogen:

Recombinant Protein of ATG4b

Specificity:

The antibody detects endogenous ATG4b protein

Gene Name:

ATG4B APG4B AUTL1 KIAA0943

Protein Name:

Cysteine protease ATG4B (AUT-like 1 cysteine endopeptidase) (Autophagin-1) (Autophagy-related cysteine endopeptidase 1) (Autophagy-related protein 4 homolog B) (hAPG4B)

Other Name:

Cysteine protease ATG4B ;
AUT-like 1 cysteine endopeptidase ;
Autophagin-1 ;
Autophagy-related cysteine endopeptidase 1 ;
Autophagy-related protein 4 homolog B ;
hAPG4B ;

Database Link:

Organism	Gene ID	SwissProt
Human	23192;	Q9Y4P1;
Mouse		Q8BGE6;

Background:

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008],

Function:

enzyme regulation:Inhibited by N-ethylmaleimide.,Function:Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.,similarity:Belongs to the peptidase C54 family.,tissue specificity:Mainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas.,

Cellular Localization:

Cytoplasm . Cytoplasm, cytosol . Cytoplasmic vesicle, autophagosome . Endoplasmic reticulum . Mitochondrion . Mainly localizes to the cytoplasm, including cytosol (PubMed:29165041). A samll potion localizes to mitochondria; phosphorylation at Ser-34 promotes localization to mitochondria (PubMed:29165041). .

Tissue Expression:

Brain,Embryo,Endometrium,Epithelium,Hippocampus,Liver,Placenta,Test

Research Areas:

>>Autophagy - other ;
>>Autophagy - animal