Main Information

Target

Atg4C

Host Species

Rabbit

Reactivity

Human, Mouse, Rat

Applications

IHC, IF

MW

57kD (Observed)

Conjugate/Modification

Unmodified

Detailed Information

Recommended Dilution Ratio

IHC 1:50-300; IF 1:50-200

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Specificity

The antibody detects endogenous ATG4c protein

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

Concentration

1 mg/ml

MW(Observed)

57kD

Modification

Unmodified

Clonality

Polyclonal

Isotype

IgG

Antigen&Target Information

Immunogen:

Recombinant Protein of ATG4c

Specificity:

The antibody detects endogenous ATG4c protein

Gene Name:

ATG4C APG4C AUTL1 AUTL3

Protein Name:

Cysteine protease ATG4C (AUT-like 3 cysteine endopeptidase) (Autophagin-3) (Autophagy-related cysteine endopeptidase 3) (Autophagy-related protein 4 homolog C)

Other Name:

Cysteine protease ATG4C ;
AUT-like 3 cysteine endopeptidase ;
Autophagin-3 ;
Autophagy-related cysteine endopeptidase 3 ;
Autophagy-related protein 4 homolog C ;

Database Link:

Organism	Gene ID	SwissProt
Human	84938;	Q96DT6;
Mouse		Q811C2;

Background:

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding the same protein, have been characterized. [provided by RefSeq, Jul 2008],

Function:

enzyme regulation:Inhibited by N-ethylmaleimide.,Function:Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.,similarity:Belongs to the peptidase C54 family.,tissue specificity:Highly expressed in skeletal muscle, heart, liver and testis.,

Cellular Localization:

Cytoplasm .

Tissue Expression:

Brain,Heart,Placenta,Testis,

Research Areas:

>>Autophagy - other ;
>>Autophagy - animal