Main Information

Target

ATG4A

Host Species

Rabbit

Reactivity

Human, Mouse, Rat

Applications

IHC, IF

MW

45kD (Observed)

Conjugate/Modification

Unmodified

Detailed Information

Recommended Dilution Ratio

IHC 1:50-300; IF 1:50-200

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Specificity

The antibody detects endogenous ATG4a protein

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

Concentration

1 mg/ml

MW(Observed)

45kD

Modification

Unmodified

Clonality

Polyclonal

Isotype

IgG

Antigen&Target Information

Immunogen:

Recombinant Protein of ATG4a

Specificity:

The antibody detects endogenous ATG4a protein

Gene Name:

ATG4A APG4A AUTL2

Protein Name:

Cysteine protease ATG4A (AUT-like 2 cysteine endopeptidase) (Autophagin-2) (Autophagy-related cysteine endopeptidase 2) (Autophagy-related protein 4 homolog A) (hAPG4A)

Other Name:

Cysteine protease ATG4A ;
AUT-like 2 cysteine endopeptidase ;
Autophagin-2 ;
Autophagy-related cysteine endopeptidase 2 ;
Autophagy-related protein 4 homolog A ;
hAPG4A ;

Database Link:

Organism	Gene ID	SwissProt
Human	115201;	Q8WYN0;
Mouse		Q8C9S8;

Background:

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. [provided by RefSeq, Mar 2016],

Function:

enzyme regulation:Inhibited by N-ethylmaleimide.,Function:Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP.,similarity:Belongs to the peptidase C54 family.,tissue specificity:Widely expressed, at a low level, and the highest expression is observed in skeletal muscle and brain. Also detected in fetal liver.,

Cellular Localization:

Cytoplasm .

Tissue Expression:

Epithelium,Kidney,Ovary,Prostate,Testis,

Research Areas:

>>Autophagy - other ;
>>Autophagy - animal