Recommended Dilution Ratio
WB 1:2000-5000; IF 1:50-200
Formulation
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Specificity
The antibody detects endogenous HAO1 protein.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage
-15°C to -25°C/1 year(Do not lower than -25°C)
Immunogen:
Recombinant Protein of HAO1
Specificity:
The antibody detects endogenous HAO1 protein.
Protein Name:
Hydroxyacid oxidase 1
Other Name:
Hydroxyacid oxidase 1 ;
HAOX1 ;
Glycolate oxidase ;
GOX
Background:
This gene is one of three related genes that have 2-hydroxyacid oxidase activity yet differ in encoded protein amino acid sequence, tissue expression and substrate preference. Subcellular location of the encoded protein is the peroxisome. Specifically, this gene is expressed primarily in liver and pancreas and the encoded protein is most active on glycolate, a two-carbon substrate. The protein is also active on 2-hydroxy fatty acids. The transcript detected at high levels in pancreas may represent an alternatively spliced form or the use of a multiple near-consensus upstream polyadenylation site. [provided by RefSeq, Jul 2008],
Function:
Catalytic activity:(S)-2-hydroxy acid + O(2) = 2-oxo acid + H(2)O(2).,cofactor:FMN.,Function:Has 2-hydroxyacid oxidase activity. Most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids.,pathway:Organic acid metabolism; glycolic acid degradation; 3-phospho-D-glyceric acid from glycolic acid: step 1/4.,similarity:Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.,similarity:Contains 1 FMN hydroxy acid dehydrogenase domain.,tissue specificity:Liver.,
Cellular Localization:
Peroxisome matrix .
Tissue Expression:
Highly expressed in liver.
Research Areas:
>>Glyoxylate and dicarboxylate metabolism ;
>>Metabolic pathways ;
>>Carbon metabolism ;
>>Peroxisome
