Main Information

Target

HAO1

Host Species

Rabbit

Reactivity

Mouse, Rat

Applications

WB, IHC, IF

MW

41kD (Observed)

Conjugate/Modification

Unmodified

Detailed Information

Recommended Dilution Ratio

WB 1:2000-5000; IF 1:50-200

Formulation

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Specificity

The antibody detects endogenous HAO1 protein.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

MW(Observed)

41kD

Modification

Unmodified

Clonality

Polyclonal

Isotype

IgG

Antigen&Target Information

Immunogen:

Recombinant Protein of HAO1

Specificity:

The antibody detects endogenous HAO1 protein.

Gene Name:

HAO1

Protein Name:

Hydroxyacid oxidase 1

Other Name:

Hydroxyacid oxidase 1 ;
HAOX1 ;
Glycolate oxidase ;
GOX

Database Link:

Organism	Gene ID	SwissProt
Human	54363;	Q9UJM8;
Mouse		Q9WU19;

Background:

This gene is one of three related genes that have 2-hydroxyacid oxidase activity yet differ in encoded protein amino acid sequence, tissue expression and substrate preference. Subcellular location of the encoded protein is the peroxisome. Specifically, this gene is expressed primarily in liver and pancreas and the encoded protein is most active on glycolate, a two-carbon substrate. The protein is also active on 2-hydroxy fatty acids. The transcript detected at high levels in pancreas may represent an alternatively spliced form or the use of a multiple near-consensus upstream polyadenylation site. [provided by RefSeq, Jul 2008],

Function:

Catalytic activity:(S)-2-hydroxy acid + O(2) = 2-oxo acid + H(2)O(2).,cofactor:FMN.,Function:Has 2-hydroxyacid oxidase activity. Most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids.,pathway:Organic acid metabolism; glycolic acid degradation; 3-phospho-D-glyceric acid from glycolic acid: step 1/4.,similarity:Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.,similarity:Contains 1 FMN hydroxy acid dehydrogenase domain.,tissue specificity:Liver.,

Cellular Localization:

Peroxisome matrix .

Tissue Expression:

Highly expressed in liver.

Research Areas:

>>Glyoxylate and dicarboxylate metabolism ;
>>Metabolic pathways ;
>>Carbon metabolism ;
>>Peroxisome