Main Information

Target

HIF-1β

Host Species

Rabbit

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF

MW

87kD (Observed)

Conjugate/Modification

Unmodified

Detailed Information

Recommended Dilution Ratio

WB 1:1000-2000; IHC 1:200-500; IF 1:50-200

Formulation

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Specificity

The antibody detects endogenous HIF-1β protein.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

MW(Observed)

87kD

Modification

Unmodified

Clonality

Polyclonal

Isotype

IgG

Antigen&Target Information

Immunogen:

Recombinant Protein of HIF-1β

Specificity:

The antibody detects endogenous HIF-1β protein.

Gene Name:

ARNT

Protein Name:

Aryl hydrocarbon receptor nuclear translocator

Other Name:

ARNT ;
BHLHE2 ;
Aryl hydrocarbon receptor nuclear translocator ;
ARNT protein ;
Class E basic helix-loop-helix protein 2 ;
bHLHe2 ;
Dioxin receptor, nuclear translocator ;
Hypoxia-inducible factor 1-beta ;
HIF-1-beta ;
HIF1-beta

Database Link:

Organism	Gene ID	SwissProt
Human	405;	P27540;
Mouse		P53762;
Rat		P41739;

Background:

This gene encodes a protein containing a basic helix-loop-helix domain and two characteristic PAS domains along with a PAC domain. The encoded protein binds to ligand-bound aryl hydrocarbon receptor and aids in the movement of this complex to the nucleus, where it promotes the expression of genes involved in xenobiotic metabolism. This protein is also a co-factor for transcriptional regulation by hypoxia-inducible factor 1. Chromosomal translocation of this locus with the ETV6 (ets variant 6) gene on chromosome 12 have been described in leukemias. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2013],

Function:

Function:Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia.,similarity:Contains 1 basic helix-loop-helix (bHLH) domain.,similarity:Contains 1 PAC (PAS-associated C-terminal) domain.,similarity:Contains 2 PAS (PER-ARNT-SIM) domains.,subunit:Efficient DNA binding requires dimerization with another bHLH protein. Forms a heterodimer with AHR, AHRR, HIF1A and EPAS1/HIF2A as well as with other bHLH proteins. Interacts with TACC3 (By similarity). Interacts with NOCA7.,

Cellular Localization:

Nucleus.

Tissue Expression:

Aorta endothelial cell,Brain,Kidney,Thalamus,Uterus,

Research Areas:

>>HIF-1 signaling pathway ;
>>Cushing syndrome ;
>>Pathways in cancer ;
>>Chemical carcinogenesis - receptor activation ;
>>Chemical carcinogenesis - reactive oxygen species ;
>>Renal cell carcinoma