Main Information

Target

GST-Tag

Host Species

Rabbit

Reactivity

Species independent

Applications

WB, ELISA

Conjugate/Modification

Unmodified

Detailed Information

Recommended Dilution Ratio

WB 1:5000-10000; ELISA 1:5000-20000

Formulation

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Specificity

The antibody detects C-terminal, internal, and N-terminal GST fusion proteins.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

Modification

Unmodified

Clonality

Polyclonal

Isotype

IgG

Antigen&Target Information

Immunogen:

Recombinant Protein of GST-Tag

Specificity:

The antibody detects C-terminal, internal, and N-terminal GST fusion proteins.

Other Name:

YM3205

Background:

GST (Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. Antibodies to GST are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in cells.