Main Information

Target

PDGFR-β

Host Species

Rabbit

Reactivity

Human, Mouse, Rat

Applications

IHC, IF, WB

MW

135-180kD (Observed)

Conjugate/Modification

Phospho

Detailed Information

Recommended Dilution Ratio

IHC 1:50-200; WB 1:500-2000; IF 1:50-200

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Specificity

This antibody detects endogenous phospho levels of PDGFRb (Phospho-Tyr857) at Human:Y857, Mouse:Y856, Rat:Y856

Purification

The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

Concentration

1 mg/ml

MW(Observed)

135-180kD

Modification

Phospho

Clonality

Polyclonal

Isotype

IgG

Antigen&Target Information

Immunogen:

Synthesized peptide derived from human PDGFRb (Phospho-Tyr857)

Specificity:

This antibody detects endogenous phospho levels of PDGFRb (Phospho-Tyr857) at Human:Y857, Mouse:Y856, Rat:Y856

Gene Name:

PDGFRB PDGFR PDGFR1

Protein Name:

PDGFRb (Phospho-Tyr857)

Other Name:

Platelet-derived growth factor receptor beta ;
PDGF-R-beta ;
PDGFR-beta ;
Beta platelet-derived growth factor receptor ;
Beta-type platelet-derived growth factor receptor ;
CD140 antigen-like family member B ;
Platelet-derived growth factor receptor 1 ;
PDGFR-1 ;
CD antigen CD140b ;

Database Link:

Organism	Gene ID	SwissProt
Human	5159;	P09619;

Background:

This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the translocation, ETV6, leukemia gene, results in chronic myeloproliferative disorder with eosinophilia. [provided by RefSeq, Jul 2008],

Function:

Catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,Disease:A chromosomal aberration involving PDGFRB is a cause in many instances of chronic myeloproliferative disorder with eosinophilia (MPE) [MIM:131440]. Translocation t(5;12) with ETV6 on chromosome 12 creating an PDGFRB-ETV6 fusion protein.,Disease:A chromosomal aberration involving PDGFRB is found in a form of chronic myelomonocytic leukemia (CMML). Translocation t(5;12)(q33;p13) with EVT6/TEL. It is characterized by abnormal clonal myeloid proliferation and by progression to acute myelogenous leukemia (AML).,Disease:A chromosomal aberration involving PDGFRB may be a cause of acute myelogenous leukemia. Translocation t(5;14)(q33;q32) with TRIP11. The fusion protein may be involved in clonal evolution of leukemia and eosinophilia.,Disease:A chromosomal aberration involving PDGFRB may be a cause of juvenile myelomonocytic leukemia. Translocation t(5;17)(q33;p11.2) with SPECC1.,Disease:A chromosomal aberration involving PDGFRB may be the cause of a myeloproliferative disorder (MBD) associated with eosinophilia. Translocation t(1;5)(q23;q33) that forms a PDE4DIP-PDGFRB fusion protein.,Function:Receptor that binds specifically to PDGFB and PDGFD and has a tyrosine-protein kinase activity. Phosphorylates Tyr residues at the C-terminus of PTPN11 creating a binding site for the SH2 domain of GRB2.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 5 Ig-like C2-type (immunoglobulin-like) domains.,subunit:Homodimer, and heterodimer with PDGFRA. Interacts with APS. The autophosphorylated form interacts directly with SHB and with PIK3C2B, maybe indirectly.,

Cellular Localization:

Cell membrane; Single-pass type I membrane protein. Cytoplasmic vesicle. Lysosome lumen. After ligand binding, the autophosphorylated receptor is ubiquitinated and internalized, leading to its degradation.

Tissue Expression:

Brain,Spleen,

Research Areas:

>>EGFR tyrosine kinase inhibitor resistance ;
>>MAPK signaling pathway ;
>>Ras signaling pathway ;
>>Rap1 signaling pathway ;
>>Calcium signaling pathway ;
>>Phospholipase D signaling pathway ;
>>PI3K-Akt signaling pathway ;
>>Focal adhesion ;
>>Gap junction ;
>>JAK-STAT signaling pathway ;
>>Regulation of actin cytoskeleton ;
>>Human papillomavirus infection ;
>>Pathways in cancer ;
>>MicroRNAs in cancer ;
>>Glioma ;
>>Prostate cancer ;
>>Melanoma ;
>>Central carbon metabolism in cancer ;
>>Choline metabolism in cancer