Main Information

Target

ACAP1

Host Species

Rabbit

Reactivity

Human, Rat, Mouse,

Applications

WB, IHC, IF, ELISA

MW

82kD (Calculated)

Conjugate/Modification

Phospho

Detailed Information

Recommended Dilution Ratio

WB 1:500-2000; WB 1:500-2000; IHC 1:100-1:300; ELISA 1:10000-40000; IF 1:50-200

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Specificity

Phospho-ACAP1 (S554) Polyclonal Antibody detects endogenous levels of ACAP1 protein only when phosphorylated at S554.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

Concentration

1 mg/ml

MW(Calculated)

82kD

Modification

Phospho

Clonality

Polyclonal

Isotype

IgG

Antigen&Target Information

Immunogen:

The antiserum was produced against synthesized peptide derived from human Centaurin-beta1 around the phosphorylation site of Ser554. AA range:520-569

Specificity:

Phospho-ACAP1 (S554) Polyclonal Antibody detects endogenous levels of ACAP1 protein only when phosphorylated at S554.

Gene Name:

ACAP1

Protein Name:

Arf-GAP with coiled-coil ANK repeat and PH domain-containing protein 1

Other Name:

ACAP1 ;
CENTB1 ;
KIAA0050 ;
Arf-GAP with coiled-coil ;
ANK repeat and PH domain-containing protein 1 ;
Centaurin-beta-1 ;
Cnt-b1

Database Link:

Organism	Gene ID	SwissProt
Human	9744;	Q15027;
Mouse		Q8K2H4;

Background:

domain:PH domain binds phospholipids including phosphatidic acid, phosphatidylinositol 3-phosphate, phosphatidylinositol 3,5-bisphosphate (PIP2) and phosphatidylinositol 3,4,5-trisphosphate (PIP3). May mediate ACAP1-binding to PIP2 or PIP3 containing membranes.,enzyme regulation:GAP activity stimulated by phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphatidic acid.,function:GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface.,miscellaneous:Cells overexpressing ACAP1 show an accumulation of ITGB1 in recycling endosomes and inhibition of stimulation-dependent cell migration. Cells with reduced levels of ACAP1 or AKT1 and AKT2 show inhibition of stimulation-dependent cell migration. Cells overexpressing ACAP1 and PIP5K1C show formation of tubular structures derived from endosomal membranes.,PTM:Phosphorylation at Ser-554 by PKB is required for interaction with ITGB1, export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.,similarity:Contains 1 Arf-GAP domain.,similarity:Contains 1 BAR domain.,similarity:Contains 1 PH domain.,similarity:Contains 3 ANK repeats.,subunit:Interacts with GTP-bound ARF6. Interacts with third cytoplasmic loop of SLC2A4/GLUT4. Interacts with CLTC. Interacts with GULP1. Forms a complex with GDP-bound ARF6 and GULP1.,tissue specificity:Highest level in lung and spleen. Low level in heart, kidney, liver and pancreas.,

Function:

Domain:PH domain binds phospholipids including phosphatidic acid, phosphatidylinositol 3-phosphate, phosphatidylinositol 3,5-bisphosphate (PIP2) and phosphatidylinositol 3,4,5-trisphosphate (PIP3). May mediate ACAP1-binding to PIP2 or PIP3 containing membranes.,enzyme regulation:GAP activity stimulated by phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphatidic acid.,Function:GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface.,miscellaneous:Cells overexpressing ACAP1 show an accumulation of ITGB1 in recycling endosomes and inhibition of stimulation-dependent cell migration. Cells with reduced levels of ACAP1 or AKT1 and AKT2 show inhibition of stimulation-dependent cell migration. Cells overexpressing ACAP1 and PIP5K1C show formation of tubular structures derived from endosomal membranes.,PTM:Phosphorylation at Ser-554 by PKB is required for interaction with ITGB1, export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.,similarity:Contains 1 Arf-GAP domain.,similarity:Contains 1 BAR domain.,similarity:Contains 1 PH domain.,similarity:Contains 3 ANK repeats.,subunit:Interacts with GTP-bound ARF6. Interacts with third cytoplasmic loop of SLC2A4/GLUT4. Interacts with CLTC. Interacts with GULP1. Forms a complex with GDP-bound ARF6 and GULP1.,tissue specificity:Highest level in lung and spleen. Low level in heart, kidney, liver and pancreas.,

Cellular Localization:

Recycling endosome membrane ; Peripheral membrane protein ; Cytoplasmic side .

Tissue Expression:

Highest level in lung and spleen. Low level in heart, kidney, liver and pancreas.

Research Areas:

>>Endocytosis