Main Information

Target

eIF5A/eIF5A2

Host Species

Rabbit

Reactivity

Human, Mouse, Rat

Applications

WB, ELISA

MW

17kD (Observed)

Conjugate/Modification

Unmodified

Detailed Information

Recommended Dilution Ratio

WB 1:500-2000; ELISA(peptide)1:5000-20000

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Specificity

This antibody detects endogenous levels of human eIF5A/eIF5A2

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

Concentration

1 mg/ml

MW(Observed)

17kD

Modification

Unmodified

Clonality

Polyclonal

Isotype

IgG

Antigen&Target Information

Immunogen:

Synthesized peptide derived from human eIF5A/eIF5A2

Specificity:

This antibody detects endogenous levels of human eIF5A/eIF5A2

Gene Name:

EIF5A

Protein Name:

eIF5A/eIF5A2

Other Name:

Eukaryotic translation initiation factor 5A-1 ;
eIF-5A-1 ;
eIF-5A1 ;
Eukaryotic initiation factor 5A isoform 1 ;
eIF-5A ;
Rev-binding factor ;
eIF-4D ;

Database Link:

Organism	Gene ID	SwissProt
Human	1984;	P63241;Q9GZV4;

Function:

Function:The precise role of eIF-5A in protein biosynthesis is not known but it functions by promoting the formation of the first peptide bond.,PTM:eIF-5A seems to be the only eukaryotic protein to have an hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine).,similarity:Belongs to the eIF-5A family.,subunit:Found in a complex with Ran and XPO4. The hypusine modification increases the interaction with XPO4.,tissue specificity:Expressed in umbilical vein endothelial cells and several cancer cell lines (at protein level).,

Cellular Localization:

Cytoplasm . Nucleus . Endoplasmic reticulum membrane ; Peripheral membrane protein ; Cytoplasmic side . Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions (PubMed:19379712, PubMed:27306458). Nuclear export of hypusinated protein is mediated by XPO4 (PubMed:10944119, PubMed:27306458). .

Tissue Expression:

Expressed in umbilical vein endothelial cells and several cancer cell lines (at protein level).