Main Information

Target

Glutaredoxin-1

Host Species

Rabbit

Reactivity

Human, Rat, Mouse,

Applications

WB, IHC, IF, ELISA

MW

12kD (Calculated)

Conjugate/Modification

Unmodified

Detailed Information

Recommended Dilution Ratio

WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:5000; Not yet tested in other applications.

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Specificity

This antibody detects endogenous levels of human Glutaredoxin-1

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

Concentration

1 mg/ml

MW(Calculated)

12kD

Modification

Unmodified

Clonality

Polyclonal

Isotype

IgG

Antigen&Target Information

Immunogen:

Synthesized peptide derived from human Glutaredoxin-1 AA range: 8-58

Specificity:

This antibody detects endogenous levels of human Glutaredoxin-1

Gene Name:

GLRX GRX

Protein Name:

Glutaredoxin-1

Other Name:

Glutaredoxin-1 ;
Thioltransferase-1 ;
TTase-1 ;

Database Link:

Organism	Gene ID	SwissProt
Human	2745;	P35754;

Background:

This gene encodes a member of the glutaredoxin family. The encoded protein is a cytoplasmic enzyme catalyzing the reversible reduction of glutathione-protein mixed disulfides. This enzyme highly contributes to the antioxidant defense system. It is crucial for several signalling pathways by controlling the S-glutathionylation status of signalling mediators. It is involved in beta-amyloid toxicity and Alzheimer's disease. Multiple alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq, Aug 2011],

Function:

Function:Has a glutathione-disulfide oxidoreductase activity in the presence of NADPH and glutathione reductase. Reduces low molecular weight disulfides and proteins.,similarity:Belongs to the glutaredoxin family.,similarity:Contains 1 glutaredoxin domain.,

Cellular Localization:

Cytoplasm.

Tissue Expression:

Blood,Brain,Lens,Liver,Skin,Spleen,Urinary bladder,