Main Information

Target

POLD4

Host Species

Rabbit

Reactivity

Human, Mouse

Applications

WB, IHC, IF, ELISA

MW

12kD (Calculated)

Conjugate/Modification

Unmodified

Detailed Information

Recommended Dilution Ratio

WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:5000; Not yet tested in other applications.

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Specificity

This antibody detects endogenous levels of human POLD4

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

Concentration

1 mg/ml

MW(Calculated)

12kD

Modification

Unmodified

Clonality

Polyclonal

Isotype

IgG

Antigen&Target Information

Immunogen:

Synthesized peptide derived from human POLD4 AA range: 43-93

Specificity:

This antibody detects endogenous levels of human POLD4

Gene Name:

POLD4 POLDS

Protein Name:

POLD4

Other Name:

DNA polymerase delta subunit 4 ;
DNA polymerase delta subunit p12 ;

Database Link:

Organism	Gene ID	SwissProt
Human	57804;	Q9HCU8;

Background:

This gene encodes the smallest subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. The encoded protein enhances the activity of DNA polymerase delta and plays a role in fork repair and stabilization through interactions with the DNA helicase Bloom syndrome protein. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012],

Function:

Function:Required for optimal DNA polymerase delta activity. May contribute to PCNA-dependent activity of DNA polymerase delta.,similarity:Belongs to the DNA polymerase delta subunit 4 family.,subunit:Heterotetramer composed of subunits of 125 kDa, 50 kDa, 66 kDa and 12 kDa. Interacts with WRNIP1. Interacts with POLD4, POLD2, POLD1 and PCNA.,

Cellular Localization:

Nucleus . Partially recruited to DNA damage sites within 2 hours following UV irradiation, before degradation. .

Tissue Expression:

Brain,Cervix,Ovarian carcinoma,Pooled,

Research Areas:

>>DNA replication ;
>>Base excision repair ;
>>Nucleotide excision repair ;
>>Mismatch repair ;
>>Homologous recombination