Main Information

Target

AATC

Host Species

Rabbit

Reactivity

Human, Mouse, Rat

Applications

IHC, IF, WB

MW

42kD (Observed)

Conjugate/Modification

Unmodified

Detailed Information

Recommended Dilution Ratio

IHC 1:50-200; WB 1:500-2000; IF 1:50-200

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Specificity

This antibody detects endogenous levels of human AATC

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

Concentration

1 mg/ml

MW(Observed)

42kD

Modification

Unmodified

Clonality

Polyclonal

Isotype

IgG

Antigen&Target Information

Immunogen:

Synthesized peptide derived from human AATC

Specificity:

This antibody detects endogenous levels of human AATC

Gene Name:

GOT1

Protein Name:

AATC

Other Name:

Aspartate aminotransferase, cytoplasmic ;
Glutamate oxaloacetate transaminase 1 ;
Transaminase A ;

Database Link:

Organism	Gene ID	SwissProt
Human	2805;	P17174;

Background:

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq, Jul 2008],

Function:

Catalytic activity:L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate.,cofactor:Pyridoxal phosphate.,miscellaneous:In eukaryotes there are cytoplasmic, mitochondrial and chloroplastic isozymes.,similarity:Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.,subunit:Homodimer.,

Cellular Localization:

Cytoplasm .

Tissue Expression:

Brain,Liver,Lung,

Research Areas:

>>Arginine biosynthesis ;
>>Alanine, aspartate and glutamate metabolism ;
>>Cysteine and methionine metabolism ;
>>Arginine and proline metabolism ;
>>Tyrosine metabolism ;
>>Phenylalanine metabolism ;
>>Phenylalanine, tyrosine and tryptophan biosynthesis ;
>>Metabolic pathways ;
>>Carbon metabolism ;
>>2-Oxocarboxylic acid metabolism ;
>>Biosynthesis of amino acids