Main Information

Target

EAAT2

Host Species

Rabbit

Reactivity

Human, Rat, Mouse

Applications

WB

MW

62kD (Observed)

Conjugate/Modification

Unmodified

Detailed Information

Recommended Dilution Ratio

WB 1:1000-2000

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Specificity

EAAT2 protein(A223) detects endogenous levels of EAAT2

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

Concentration

1 mg/ml

MW(Observed)

62kD

Modification

Unmodified

Clonality

Polyclonal

Isotype

IgG

Antigen&Target Information

Immunogen:

Synthetic Peptide of EAAT2 AA range: 370-420

Specificity:

EAAT2 protein(A223) detects endogenous levels of EAAT2

Gene Name:

SLC1A2

Protein Name:

Excitatory amino acid transporter 2 (Glutamate/aspartate transporter II) (Sodium-dependent glutamate/aspartate transporter 2) (Solute carrier family 1 member 2)

Other Name:

Excitatory amino acid transporter 2 ;
Glutamate/aspartate transporter II ;
Sodium-dependent glutamate/aspartate transporter 2 ;
Solute carrier family 1 member 2 ;

Database Link:

Organism	Gene ID	SwissProt
Human	6506;	P43004;
Mouse		P43006;
Rat		P31596;

Background:

This gene encodes a member of a family of solute transporter proteins. The membrane-bound protein is the principal transporter that clears the excitatory neurotransmitter glutamate from the extracellular space at synapses in the central nervous system. Glutamate clearance is necessary for proper synaptic activation and to prevent neuronal damage from excessive activation of glutamate receptors. Mutations in and decreased expression of this protein are associated with amyotrophic lateral sclerosis. Alternatively spliced transcript variants of this gene have been identified. [provided by RefSeq, Sep 2010],

Function:

Function:Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.,PTM:Glycosylated.,similarity:Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family.,subunit:Homotrimer. Interacts with JUB.,

Cellular Localization:

Cell membrane ; Multi-pass membrane protein .

Tissue Expression:

Brain,Brain cortex,Pancreas,

Research Areas:

>>Synaptic vesicle cycle ;
>>Glutamatergic synapse ;
>>Amyotrophic lateral sclerosis ;
>>Huntington disease