Main Information

Target

GABA A Receptor α4

Host Species

Rabbit

Reactivity

Human, Rat, Mouse

Applications

WB, IHC, IF

MW

62kD (Observed)

Conjugate/Modification

Unmodified

Detailed Information

Recommended Dilution Ratio

WB 1:1000-2000; IHC 1:100-200; IF 1:50-200

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Specificity

GABA A Receptor α4 protein(A226) detects endogenous levels of GABA A Receptor α4

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

Concentration

1 mg/ml

MW(Observed)

62kD

Modification

Unmodified

Clonality

Polyclonal

Isotype

IgG

Antigen&Target Information

Immunogen:

Synthetic Peptide of GABA A Receptor α4 AA range: 149-199

Specificity:

GABA A Receptor α4 protein(A226) detects endogenous levels of GABA A Receptor α4

Gene Name:

GABRA4

Protein Name:

Gamma-aminobutyric acid receptor subunit alpha-4 (GABA(A) receptor subunit alpha-4)

Other Name:

GABRA4 ;
Gamma-aminobutyric acid receptor subunit alpha-4 ;
GABA ;
A ;
receptor subunit alpha-4

Database Link:

Organism	Gene ID	SwissProt
Human	2557;	P48169;
Mouse		Q9D6F4;
Rat		P28471;

Background:

Gamma-aminobutyric acid (GABA) is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified. This gene encodes subunit alpha-4, which is involved in the etiology of autism and eventually increases autism risk through interaction with another subunit, gamma-aminobutyric acid receptor beta-1 (GABRB1). Alternatively spliced transcript variants encoding different isoforms have been found in this gene.[provided by RefSeq, Feb 2011],

Function:

Function:GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.,induction:The alpha4 beta2 gamma 2L receptor is not repressed by diazepam.,online information:Forbidden fruit - Issue 56 of March 2005,similarity:Belongs to the ligand-gated ionic channel (TC 1.A.9) family.,subunit:Generally pentameric. There are five types of GABA(A) receptor chains: alpha, beta, gamma, delta, and rho.,

Cellular Localization:

Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.

Tissue Expression:

Brain,Brain cortex,

Research Areas:

>>Neuroactive ligand-receptor interaction ;
>>Retrograde endocannabinoid signaling ;
>>GABAergic synapse ;
>>Taste transduction ;
>>Morphine addiction ;
>>Nicotine addiction