Main Information

Target

SLC12A4

Host Species

Rabbit

Reactivity

Human, Rat, Mouse

Applications

WB, IHC, IF

MW

150kD (Observed)

Conjugate/Modification

Unmodified

Detailed Information

Recommended Dilution Ratio

WB 1:1000-2000; IHC 1:100-200; IF 1:50-200

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Specificity

SLC12A4 protein(A248) detects endogenous levels of SLC12A4

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

Concentration

1 mg/ml

MW(Observed)

150kD

Modification

Unmodified

Clonality

Polyclonal

Isotype

IgG

Antigen&Target Information

Immunogen:

Synthetic Peptide of SLC12A4 AA range: 658-708

Specificity:

SLC12A4 protein(A248) detects endogenous levels of SLC12A4

Gene Name:

SLC12A4

Protein Name:

Solute carrier family 12 member 4 (Electroneutral potassium-chloride cotransporter 1) (Erythroid K-Cl cotransporter 1) (hKCC1)

Other Name:

Solute carrier family 12 member 4 ;
Electroneutral potassium-chloride cotransporter 1 ;
Erythroid K-Cl cotransporter 1 ;
hKCC1 ;

Database Link:

Organism	Gene ID	SwissProt
Human	6560;	Q9UP95;
Mouse		Q9JIS8;
Rat		Q63632;

Background:

This gene encodes a member of the SLC12A transporter family. The encoded protein mediates the coupled movement of potassium and chloride ions across the plasma membrane. This gene is expressed ubiquitously. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jan 2013],

Function:

Alternative products:Experimental confirmation may be lacking for some isoforms,Function:Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May contribute to cell volume homeostasis in single cells. May be involved in the regulation of basolateral Cl(-) exit in NaCl absorbing epithelia (By similarity). Isoform 4 has no transport activity.,PTM:N-glycosylated.,similarity:Belongs to the SLC12A transporter family.,subunit:Homomultimer and heteromultimer with other K-Cl cotransporters.,tissue specificity:Ubiquitous. Levels are much higher in erythrocytes from patients with Hb SC and Hb SS compared to normal AA erythrocytes. This may contribute to red blood cell dehydration and to the manifestation of sickle cell disease by increasing the intracellular concentration of HbS. Isoform 1 was not detected in circulating reticulocytes.,

Cellular Localization:

Membrane; Multi-pass membrane protein.

Tissue Expression:

Ubiquitous. Levels are much higher in erythrocytes from patients with Hb SC and Hb SS compared to normal AA erythrocytes. This may contribute to red blood cell dehydration and to the manifestation of sickle cell disease by increasing the intracellular concentration of HbS. Isoform 1 was not detected in circulating reticulocytes.