Main Information

Target

LC3B

Host Species

Rabbit

Reactivity

Human, Mouse, Rat

Applications

WB, IF, IHC

MW

14,16kD (Observed)

Conjugate/Modification

Unmodified

Detailed Information

Recommended Dilution Ratio

WB 1:1000-2000; IHC 1:200-500; IF 1:200

Formulation

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Specificity

The antibody detects endogenous MAP LC3β protein.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

MW(Observed)

14,16kD

Modification

Unmodified

Clonality

Polyclonal

Isotype

IgG

Antigen&Target Information

Immunogen:

Recombinant Protein of MAP LC3β

Specificity:

The antibody detects endogenous MAP LC3β protein.

Gene Name:

MAP1LC3B

Protein Name:

Microtubule-associated proteins 1A/1B light chain 3B

Other Name:

MAP1LC3B ;
MAP1ALC3 ;
Microtubule-associated proteins 1A/1B light chain 3B ;
Autophagy-related protein LC3 B ;
Autophagy-related ubiquitin-like modifier LC3 B ;
MAP1 light chain 3-like protein 2 ;
MAP1A/MAP1B light chain 3 B ;
MAP1A/MAP1B LC3 B ;
Microtubule-associated protein 1 light chain 3 beta

Database Link:

Organism	Gene ID	SwissProt
Human	81631;	Q9GZQ8;
Mouse		Q9CQV6;
Rat		Q62625;

Background:

The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq, Jul 2008],

Function:

Caution:PubMed:12740394 has shown that the protein is cleaved at Lys-122 but PubMed:15355958 has shown that the cleavage site is at Gly-120 as in other mammalian orthologs.,Function:Probably involved in formation of autophagosomal vacuoles (autophagosomes).,PTM:The precursor molecule is cleaved by APG4B/ATG4B to form LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form LC3-II.,similarity:Belongs to the MAP1 LC3 family.,subcellular location:LC3-II binds to the autophagic membranes.,subunit:3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins.,tissue specificity:Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver.,

Cellular Localization:

Cytoplasmic vesicle, autophagosome membrane ; Lipid-anchor . Endomembrane system ; Lipid-anchor . Mitochondrion membrane ; Lipid-anchor . Cytoplasm, cytoskeleton . Cytoplasmic vesicle . LC3-II binds to the autophagic membranes. LC3-II localizes with the mitochondrial inner membrane during Parkin-mediated mitophagy (PubMed:28017329). Localizes also to discrete punctae along the ciliary axoneme. .

Tissue Expression:

Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver.

Research Areas:

>>Mitophagy - animal ;
>>Autophagy - animal ;
>>Ferroptosis ;
>>Apelin signaling pathway ;
>>NOD-like receptor signaling pathway ;
>>Amyotrophic lateral sclerosis ;
>>Pathways of neurodegeneration - multiple diseases ;
>>Shigellosis ;
>>Kaposi sarcoma-associated herpesvirus infection