English
   中文版
邮箱: sinaone_vip@163.com    电话: 01056545366
首页 启研抗体 磷酸化抗体
<
  •  Immunofluorescence analysis of mouse-liver tissue. 1,GSK3α/β (phospho Tyr279/216) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  •  Immunofluorescence analysis of mouse-liver tissue. 1,GSK3α/β (phospho Tyr279/216) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  •  Western Blot analysis of various cells using primary antibody diluted at 1:1000(4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour). Cell lysate was extracted by Minute™ Plasma Membrane Protein Isolation and Cell Fractionation Kit(SM-005, Inventbiotech,MN,USA).
  •  Western Blot analysis of various cells using primary antibody diluted at 1:1000(4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour). Cell lysate was extracted by Minute™ Plasma Membrane Protein Isolation and Cell Fractionation Kit(SM-005, Inventbiotech,MN,USA).
  •  Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,GSK3α/β (phospho Tyr279/216) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
>
GSK3α/β (phospho Tyr279/216) Polyclonal Antibody 
   目录号 QYA04229    
规格
目录价
促销价
库存
购买
100ug/100ul 2250.00 1350.00
25
   
图片描述:
100ug/100ul
文件与质量管理
COA Datasheet
商品基本信息:

Catalog No.

QYA04229

Size.

100ug

Gene Name.

GSK3A/GSK3B

Protein Name.

Glycogen synthase kinase-3 alpha/beta

Source.

Rabbit

Immunogen.

Synthesized phospho-peptide around the phosphorylation site of human GSK3α/β (phospho Tyr279/216)

Purification.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity.

Phospho-GSK3α/β (Y279/216) Polyclonal Antibody detects endogenous levels of GSK3α/β protein only when phosphorylated at Y279/216.

Formulation.

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration.

1 mg/ml

Storage / Stability.

-20°C/1 year

Human Gene ID.

2931

Human Swiss-Prot No.

P49840/P49841

Mouse Swiss-Prot No.

 

Rat Swiss-Prot No.

P18265/P18266

Reactivity.

Human, Mouse, Rat

Applications.

IF WB,IHC-p,ELISA

Predicted band (Da)

50981

Observed band (KDa)

51,46

Dilution.

IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.

Other name.

GSK3A; Glycogen synthase kinase-3 alpha; GSK-3 alpha; Serine/threonine-protein kinase GSK3A; GSK3B; Glycogen synthase kinase-3 beta; GSK-3 beta; Serine/threonine-protein kinase GSK3B

其他技术信息

更多信息请下载和查阅产品说明书。

请把您的问题发送给我们,感谢您的支持!