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  •  Immunofluorescence analysis of rat-liver tissue. 1,Akt (phospho Ser473) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  •  Immunofluorescence analysis of rat-liver tissue. 1,Akt (phospho Ser473) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  •  Western Blot analysis of 3T3 cells treated with PDGF using primary antibody diluted at 1:1000(4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour)
  •  Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,Akt (phospho Ser473) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
  •  Western Blot analysis of various cells using Phospho-Akt (S473) Polyclonal Antibody diluted at 1:2000
  •  The picture was kindly provided by our customer
  •  Western Blot analysis of KB using Phospho-Akt (S473) Polyclonal Antibody diluted at 1:2000
  •  Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Akt (Phospho-Ser473) Antibody. The picture on the right is blocked with the phospho peptide.
  •  Western blot analysis of lysates from HeLa cells treated with heat shock, using Akt (Phospho-Ser473) Antibody. The lane on the left is blocked with the phospho peptide.
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Akt (phospho Ser473) Polyclonal Antibody 
   目录号 QYA00300A    
规格
目录价
促销价
库存
购买
100µg/100µl 2250.00 1350.00
5
   
图片描述:
100µg/100µl
文件与质量管理
COA Datasheet
商品基本信息:

Catalog No.

QYA00300A

Size.

100ug

Gene Name.

AKT1/AKT2/AKT3

Protein Name

RAC-alpha serine/threonine-protein kinase/RAC-beta serine/threonine-protein kinase/RAC-gamma serine/threonine-protein kinase

Source.

Rabbit

Immunogen.

Synthesized phospho-peptide around the phosphorylation site of human Akt (phospho Ser473)

Purification.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity.

Phospho-Akt (S473) Polyclonal Antibody detects endogenous levels of Akt protein only when phosphorylated at S473.

Formulation.

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide..

Concentration.

1 mg/ml

Storage / Stability.

-20°C/1 year

Human Gene ID.

207/208/10000

Human Swiss-Prot No.

P31749/P31751/Q9Y243

Mouse Gene ID

11651/11652/23797

Rat Gene ID

24185/25233/29414

Rat Swiss-Prot No.

P47196/P47197/Q63484

Reactivity.

Human, Mouse, Rat

Applications.

IF WB,IHC-p,ELISA

Predicted band (Da)

55716

Observed band (KDa)

55

Dilution.

IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.

Other Name

AKT1; PKB; RAC; RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha; AKT2; RAC-beta serine/threonine-protein kinase; Protein kinase Akt-2; Protein kinase B

其他技术信息

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