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  •  Western Blot analysis of various cells using Phospho-Akt1 (T450) Polyclonal Antibody diluted at 1:1000
  •  Western Blot analysis of 453 cells using Phospho-Akt1 (T450) Polyclonal Antibody diluted at 1:1000
  •  Western Blot analysis of HEPG2 KB A549 using Phospho-Akt1 (T450) Polyclonal Antibody diluted at 1:1000
  •  Western blot analysis of various lysis using Phospho-Akt1 (T450) Polyclonal Antibody diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
  •  The picture was kindly provided by our customer,antibody was diluted at 1:500
  •  Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  •  Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using Akt1 (Phospho-Thr450) Antibody. The picture on the right is blocked with the phospho peptide.
  •  Western blot analysis of lysates from NIH/3T3 cells treated with PDGF 50ng/ml 20', using Akt1 (Phospho-Thr450) Antibody. The lane on the right is blocked with the phospho peptide.
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Akt1 (phospho Thr450) Polyclonal Antibody 
   目录号 QYA00311    
规格
目录价
促销价
库存
购买
100µg/100µl 2250.00 1350.00
1
   
图片描述:
100µg/100µl
文件与质量管理
COA Datasheet
商品基本信息:

Catalog No.

QYA00311

Size.

100µg/100µl

Gene Name.

AKT1

Protein Name.

RAC-alpha serine/threonine-protein kinase

Source.

Rabbit

Immunogen.

Synthesized phospho-peptide around the phosphorylation site of human Akt1 (phospho Thr450)

Purification.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity.

Phospho-Akt1 (T450) Polyclonal Antibody detects endogenous levels of Akt1 protein only when phosphorylated at T450.

Formulation.

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration.

1 mg/ml

Storage / Stability.

-20°C/1 year

Human Gene ID.

207

Human Swiss-Prot No.

P31749

Mouse Gene ID

 

Mouse Swiss-Prot No.

P31750

Rat Gene ID

 

Rat Swiss-Prot No.

P47196

Reactivity.

Human, Mouse, Rat

Applications.

WB,IHC-p,ELISA

Predicted band (Da)

55686

Observed band (KDa)

55

Dilution.

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.

Other Name

AKT1; PKB; RAC; RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha

其他技术信息

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