描述：

Rosetta-gami host strains are derivatives of Origami (a K-12 strain), and are designed for enhanced cytoplasmic disulfide bond formation plus enhanced expression of eukaryotic proteins that contain codons rarely used in E. coli. These strains carry all of the characteristics of the Origami strain, plus they supply tRNA genes for AGG, AGA, AUA, CUA, CCC, GGA on a ColE1-compatible chloramphenicol-resistant plasmid. Thus the Rosetta-gami strains provide for "universal" translation which is otherwise limited by the codon usage of E. coli. Transcription of the tRNA genes is driven by their native promoters. DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG. 

详细说明：

出品公司：Novagen

载体类型：原核表达载体

关键功能：宿主菌

原核抗性：Chloramphenicol，Kanamycin，Streptomycin，Tetracycline