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pET-29a(+) 
   目录号 QYV0646    
规格
目录价
促销价
库存
购买
20 reactions 1200.00 1200.00
3
   
图片描述:
20 reactions
文件与质量管理
COA Datasheet
商品基本信息:

产品简介:

The pET-29a(+) vectors carry an N-terminal S•Tag™/thrombin configuration plus an optional C-terminal His•Tag® sequence. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand. Therefore, single-stranded sequencing should be performed using the T7 terminator primer.

 

产品参数:

质粒类型:原核表达载体

出品公司:Novagen

表达水平:高

克隆方法:多克隆位点,限制性内切酶

载体大小:5371 bp

5' 测序引物:T7

3' 测序引物:T7-ter

载体标签:N-S, N-Thrombin, C-His

载体抗性:Kanamycin (卡那霉素)

备注:N term thrombin cleavage site; a,b,c vary by MCS

 

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