载体介绍：

The pNTAP expression vector is used to clone a gene of interest with the SBP and CBP affinity tags adjacent to the 5´ end of the gene. The pNTAP vector is available in three different reading frames to simplify subcloning. These reading frames, designated as A, B, and C, differ only by one or two bases. Thus, each pNTAP vector has a reading frame that will allow cloning a gene of interest so that it is fused correctly with the affinity tags. The multiple cloning site (MCS) of the pNTAP vectors allows for a variety of cloning strategies, resulting in fusion of the TAP tags to the N-terminus of the protein of interest. A Kozak consensus sequence provides optimal expression of the fusion protein.

CBP tag：KRRWKKNFIAVSAANRFKKISSSGAL

SBP tag：MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREPSGGCKLG

产品参数：

质粒类型：哺乳动物表达载体

启动子：CMV

克隆方法：多克隆位点，限制性内切酶

载体大小：4531 bp 

载体标签：streptavidin-binding peptides，calmodulin-binding peptides

载体抗性：Kanamycin (卡那霉素)

筛选标记：Neomycin (新霉素)

备注：Vector for expressing proteins N-terminally tagged with calmodulin- and streptavidin-binding peptides.