载体介绍：

pcDNA3.1/myc-His(-) A, B, and C are 5.5 kb vectors designed for overproduction of recombinant proteins in mammalian cell lines. The vectors are supplied in three reading frames to facilitate in frame cloning, with a C-terminal peptide containing a polyhistidine metal-binding tag and the myc (c-myc) epitope. The human cytomegalovirus immediate-early (CMV) promoter provides highlevel expression in a wide range of mammalian cells. In addition, the vector will replicate episomally in cell lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g. COS7). High-level stable and nonreplicative transient expression can be carried out in most mammalian cells. The control plasmid, pcDNA3.1/myc-His(-)/lacZ, is the pcDNA3.1/myc-His(-) A vector with a 3.2 kb fragment containing the β-galactosidase gene cloned in frame with the C-terminal peptide. It is included for use as a positive control for transfection, expression, and detection in the cell line of choice. 

产品参数：

启动子:CMV

复制子:pUC ori，F1 ori

终止子:BGH poly（A）signal

质粒分类:哺乳细胞载体;蛋白过表达质粒

质粒大小:5522bp

质粒标签:His Tag (6x), C-Myc   Epitope Tag

原核抗性:氨苄青霉素Amp

筛选标记:新霉素Neo

克隆菌株:DH5α

培养条件:37℃，有氧，LB

表达宿主:哺乳细胞

诱导方式:IPTG或乳糖及其类似物

5'测序引物:T7   Fwd:5d[TAATACGACTCACTATAGGG]3