Main Information

Target

CD19

Host Species

Rabbit

Reactivity

Human, Mouse

Applications

IHC, WB

MW

61kD (Observed)

Conjugate/Modification

Phospho

Detailed Information

Recommended Dilution Ratio

WB 1:500-2000; IHC 1:50-200

Formulation

Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.

Specificity

This antibody detects endogenous levels of CD19 (Phospho Tyr500) Rabbit pAb at Human, Mouse

Purification

The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

Concentration

1 mg/ml

MW(Observed)

61kD

Modification

Phospho

Clonality

Polyclonal

Antigen&Target Information

Immunogen:

Synthesized peptide derived from human CD19 (Phospho Tyr500)

Specificity:

This antibody detects endogenous levels of CD19 (Phospho Tyr500) Rabbit pAb at Human, Mouse

Gene Name:

CD19

Protein Name:

B-lymphocyte antigen CD19 (B-lymphocyte surface antigen B4) (Differentiation antigen CD19) (T-cell surface antigen Leu-12) (CD antigen CD19)

Other Name:

B-lymphocyte antigen CD19 ;
B-lymphocyte surface antigen B4 ;
Differentiation antigen CD19 ;
T-cell surface antigen Leu-12 ;
CD antigen CD19 ;

Database Link:

Organism	Gene ID	SwissProt
Human	930;	P15391;
Mouse	12478;	P25918;

Background:

CD19 molecule(CD19) Homo sapiens Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. [provided by RefSeq, Jul 2008],

Function:

Disease:Defects in CD19 are a cause of hypogammaglobulinemia [MIM:107265].,Function:Assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.,online information:CD19 mutation db,PTM:Phosphorylated on serine and threonine upon DNA damage, probably by ATM or ATR. Phosphorylated on tyrosine following B-cell activation.,similarity:Contains 2 Ig-like C2-type (immunoglobulin-like) domains.,subunit:Forms a complex with CD21, CD81 and CD225 in the membrane of mature B cells. Interacts with VAV. Interacts with GRB2 and SOS when phosphorylated on Tyr-348 and/or Tyr-378. Interacts with PLCG2 when phosphorylated on Tyr-409.,

Cellular Localization:

Cell membrane ; Single-pass type I membrane protein . Membrane raft ; Single-pass type I membrane protein .

Tissue Expression:

Detected on marginal zone and germinal center B cells in lymph nodes (PubMed:2463100). Detected on blood B cells (at protein level) (PubMed:2463100, PubMed:16672701).

Research Areas:

>>PI3K-Akt signaling pathway ;
>>Hematopoietic cell lineage ;
>>B cell receptor signaling pathway ;
>>Epstein-Barr virus infection ;
>>Primary immunodeficiency