Main Information

Target

Histone H2B

Host Species

Rabbit

Reactivity

Human, Mouse, Rat

Applications

WB

MW

14kD (Observed)

Conjugate/Modification

Tri Methyl

Detailed Information

Recommended Dilution Ratio

WB 1:1000-2000

Formulation

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Specificity

The antibody detects endogenous Histone H2B (Tri Methyl Lys5) protein.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

MW(Observed)

14kD

Modification

Tri Methyl

Clonality

Polyclonal

Isotype

IgG

Antigen&Target Information

Immunogen:

Synthetic Peptide of Histone H2B (Tri Methyl Lys5)

Specificity:

The antibody detects endogenous Histone H2B (Tri Methyl Lys5) protein.

Gene Name:

HIST1H2BC

Protein Name:

Histone H2B type 1-A/Histone H2B type 1-B/Histone H2B type 1-C/E/F/G/I

Other Name:

H2BK5ME3 ;
HIST1H2BA ;
TSH2B ;
Histone H2B type 1-A ;
Histone H2B, testis ;
Testis-specific histone H2B ;
HIST1H2BB ;
H2BFF ;
Histone H2B type 1-B ;
Histone H2B.1 ;
Histone H2B.f ;
H2B/f ;
HIST1H2BC ;
H2BFL ;
HIST1H2BE ;
H2BFH ;
HIST1H2BF ;
H2BFG ;
HIST1H2BG ;
H2BFA ;
HIST1H2BI ;
H2BFK ;
Histone H2B type 1-C/E/F/G/I ;
Histone H2B.1 A ;
Histone H2B.a ;
H2B/a ;
Histone H2B.g ;
H2B/g ;
Histone H2B.h ;
H2B/h ;
Histone H2B.k ;
H2B/k ;
Histone H2B.l ;
H2B/l

Database Link:

Organism	Gene ID	SwissProt
Human	255626;3018;3017;8339;8343;8344;8346;8347;	Q96A08;P33778;P62807;
Mouse	319177;319178;319179;
Rat	24829;	Q00729;

Background:

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a testis/sperm-specific member of the histone H2B family. Transcripts from this gene contain a palindromic termination element. [provided by RefSeq, Aug 2015],

Function:

Function:Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.,PTM:Monoubiquitination of Lys-122 by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II.,similarity:Belongs to the histone H2B family.,subunit:The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.,tissue specificity:Transcribed exclusively in testis, and the corresponding protein is also present in mature sperm.,

Cellular Localization:

Nucleus . Chromosome .

Tissue Expression:

Mainly expressed in testis, and the corresponding protein is also present in mature sperm (at protein level). Also found in some fat cells.

Research Areas:

>>Neutrophil extracellular trap formation ;
>>Alcoholism ;
>>Viral carcinogenesis ;
>>Systemic lupus erythematosus