Main Information

Target

GRK 2

Host Species

Rabbit

Reactivity

Human, Mouse, Rat

Applications

IHC, IF, WB

MW

75kD (Observed)

Conjugate/Modification

Phospho

Detailed Information

Recommended Dilution Ratio

IHC 1:50-200; WB 1:500-2000; IF 1:50-200

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Specificity

This antibody detects endogenous phospho levels of GRK2 (Phospho-Tyr86) at Human:Y86, Mouse:Y86, Rat:Y86

Purification

The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

Concentration

1 mg/ml

MW(Observed)

75kD

Modification

Phospho

Clonality

Polyclonal

Isotype

IgG

Antigen&Target Information

Immunogen:

Synthesized peptide derived from human GRK2 (Phospho-Tyr86)

Specificity:

This antibody detects endogenous phospho levels of GRK2 (Phospho-Tyr86) at Human:Y86, Mouse:Y86, Rat:Y86

Gene Name:

ADRBK1 BARK BARK1 GRK2

Protein Name:

GRK2 (Phospho-Tyr86)

Other Name:

Beta-adrenergic receptor kinase 1 ;
Beta-ARK-1 ;
G-protein coupled receptor kinase 2 ;

Database Link:

Organism	Gene ID	SwissProt
Human	156;	P25098;

Background:

The product of this gene phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. This protein is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart. [provided by RefSeq, Jul 2008],

Function:

Catalytic activity:ATP + [beta-adrenergic receptor] = ADP + [beta-adrenergic receptor] phosphate.,Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,Function:Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them.,online information:Beta adrenergic receptor kinase entry,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 1 PH domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 RGS domain.,subunit:Interacts with GIT1 (By similarity). Interacts with, and phosphorylates chemokine-stimulated CCR5.,tissue specificity:Expressed in peripheral blood leukocytes.,

Cellular Localization:

Cytoplasm . Cell membrane . Cell junction, synapse, postsynapse . Cell junction, synapse, presynapse .

Tissue Expression:

Expressed in peripheral blood leukocytes.

Research Areas:

>>Chemokine signaling pathway ;
>>Endocytosis ;
>>Hedgehog signaling pathway ;
>>Glutamatergic synapse ;
>>Olfactory transduction ;
>>Morphine addiction