Main Information

Target

Flotillin-1

Host Species

Rabbit

Reactivity

Human, Mouse, Rat

Applications

WB, ELISA

MW

48kD (Observed)

Conjugate/Modification

Unmodified

Detailed Information

Recommended Dilution Ratio

WB 1:500-2000; ELISA(peptide)1:5000-20000

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Specificity

This antibody detects endogenous levels of human Flotillin-1

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

Concentration

1 mg/ml

MW(Observed)

48kD

Modification

Unmodified

Clonality

Polyclonal

Isotype

IgG

Antigen&Target Information

Immunogen:

Synthesized peptide derived from human Flotillin-1

Specificity:

This antibody detects endogenous levels of human Flotillin-1

Gene Name:

FLOT1

Protein Name:

Flotillin-1

Other Name:

Flotillin-1

Database Link:

Organism	Gene ID	SwissProt
Human	10211;	O75955;

Background:

This gene encodes an protein that localizes to the caveolae, which are small domains on the inner cell membranes. This protein plays a role in vesicle trafficking and cell morphology. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016],

Function:

Function:May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles.,similarity:Belongs to the band 7/mec-2 family. Flotillin subfamily.,subcellular location:Membrane-associated protein of caveolae. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.,subunit:Heterooligomeric complex of flotillin-1 and flotillin-2 and caveolin-1 and caveolin-2.,

Cellular Localization:

Cell membrane; Peripheral membrane protein . Endosome . Membrane, caveola ; Peripheral membrane protein . Melanosome . Membrane raft . Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065). Membrane-associated protein of caveola (By similarity). .

Tissue Expression:

Adipose tissue,Adrenal gland,Lung,Peripheral blood leukocyte,Umbilical cord blood,

Research Areas:

>>Insulin signaling pathway