Main Information

Target

ITK

Host Species

Rabbit

Reactivity

Human, Mouse

Applications

IHC, IF, WB

MW

72kD (Observed)

Conjugate/Modification

Unmodified

Detailed Information

Recommended Dilution Ratio

IHC 1:50-200; WB 1:500-2000; IF 1:50-200

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Specificity

This antibody detects endogenous levels of human ITK

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

Concentration

1 mg/ml

MW(Observed)

72kD

Modification

Unmodified

Clonality

Polyclonal

Isotype

IgG

Antigen&Target Information

Immunogen:

Synthesized peptide derived from human ITK AA range: 450-530

Specificity:

This antibody detects endogenous levels of human ITK

Gene Name:

ITK EMT LYK

Protein Name:

ITK

Other Name:

Tyrosine-protein kinase ITK/TSK ;
Interleukin-2-inducible T-cell kinase ;
IL-2-inducible T-cell kinase ;
Kinase EMT ;
T-cell-specific kinase ;
Tyrosine-protein kinase Lyk ;

Database Link:

Organism	Gene ID	SwissProt
Human	3702;	Q08881;

Background:

This gene encodes an intracellular tyrosine kinase expressed in T-cells. The protein contains both SH2 and SH3 domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation and differentiation. [provided by RefSeq, Jul 2008],

Function:

Catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,cofactor:Binds 1 zinc ion per subunit.,Function:Plays a role in T-cell proliferation and differentiation.,induction:By interleukin-2.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.,similarity:Contains 1 Btk-type zinc finger.,similarity:Contains 1 PH domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SH2 domain.,similarity:Contains 1 SH3 domain.,subcellular location:Localizes to cell surface receptors in the plasma membrane after stimulation with respective receptors (TCR, CD28, CD2) in T-cells.,tissue specificity:T-cell lines and natural killer cell lines.,

Cellular Localization:

Cytoplasm . Nucleus . Localizes in the vicinity of cell surface receptors in the plasma membrane after receptor stimulation.

Tissue Expression:

T-cell lines and natural killer cell lines.

Research Areas:

>>Chemokine signaling pathway ;
>>T cell receptor signaling pathway ;
>>Leukocyte transendothelial migration