Main Information

Target

PCP

Host Species

Rabbit

Reactivity

Human, Mouse

Applications

IHC, IF, WB

MW

100kD (Observed)

Conjugate/Modification

Unmodified

Detailed Information

Recommended Dilution Ratio

IHC 1:50-200; WB 1:500-2000; IF 1:50-200

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Specificity

This antibody detects endogenous levels of human PCP

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

Concentration

1 mg/ml

MW(Observed)

100kD

Modification

Unmodified

Clonality

Polyclonal

Isotype

IgG

Antigen&Target Information

Immunogen:

Synthesized peptide derived from human PCP

Specificity:

This antibody detects endogenous levels of human PCP

Gene Name:

PRCP PCP

Protein Name:

PCP

Other Name:

Lysosomal Pro-X carboxypeptidase ;
Angiotensinase C ;
Lysosomal carboxypeptidase C ;
Proline carboxypeptidase ;
Prolylcarboxypeptidase ;
PRCP ;

Database Link:

Organism	Gene ID	SwissProt
Human	5547;	P42785;

Background:

This gene encodes a member of the peptidase S28 family of serine exopeptidases. The encoded preproprotein is proteolytically processed to generate the mature lysosomal prolylcarboxypeptidase. This enzyme cleaves C-terminal amino acids linked to proline in peptides such as angiotension II, III and des-Arg9-bradykinin. The cleavage occurs at acidic pH, but the enzyme activity is retained with some substrates at neutral pH. This enzyme has been shown to be an activator of the cell matrix-associated prekallikrein. The importance of angiotension II, one of the substrates of this enzyme, in regulating blood pressure and electrolyte balance suggests that this gene may be related to essential hypertension. A pseudogene of this gene has been identified on chromosome 2. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [pr

Function:

Catalytic activity:Cleavage of a -Pro-|-Xaa bond to release a C-terminal amino acid.,Function:Cleaves C-terminal amino acids linked to proline in peptides such as angiotensin II, III and des-Arg9-bradykinin. This cleavage occurs at acidic pH, but enzymatic activity is retained with some substrates at neutral pH.,similarity:Belongs to the peptidase S28 family.,subunit:Homodimer.,tissue specificity:Highest levels in placenta, lung and liver. Also present in heart, brain, pancreas and kidney.,

Cellular Localization:

Lysosome.

Tissue Expression:

Highest levels in placenta, lung and liver. Also present in heart, brain, pancreas and kidney.

Research Areas:

>>Renin-angiotensin system ;
>>Protein digestion and absorption