Main Information

Target

IDHP

Host Species

Rabbit

Reactivity

Human, Mouse, Rat

Applications

IHC, IF, WB

MW

48kD (Observed)

Conjugate/Modification

Unmodified

Detailed Information

Recommended Dilution Ratio

IHC 1:50-200; WB 1:500-2000; IF 1:50-200

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Specificity

This antibody detects endogenous levels of human IDHP

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

Concentration

1 mg/ml

MW(Observed)

48kD

Modification

Unmodified

Clonality

Polyclonal

Isotype

IgG

Antigen&Target Information

Immunogen:

Synthesized peptide derived from human IDHP

Specificity:

This antibody detects endogenous levels of human IDHP

Gene Name:

IDH2

Protein Name:

IDHP

Other Name:

Isocitrate dehydrogenase [NADP], mitochondrial ;
IDH ;
ICD-M ;
IDP ;
NADP ;
+ ;
-specific ICDH ;
Oxalosuccinate decarboxylase ;

Database Link:

Organism	Gene ID	SwissProt
Human	3418;	P48735;

Background:

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. Alternative splicing results in multiple transcript variants. [provided by Ref

Function:

Catalytic activity:Isocitrate + NADP(+) = 2-oxoglutarate + CO(2) + NADPH.,Catalytic activity:Oxalosuccinate + NADP(+) = 2-oxoglutarate + CO(2) + NADPH.,cofactor:Binds 1 magnesium or manganese ion per subunit.,Function:Plays a role in intermediary metabolism and energy production. It may tightly associate or interact with the pyruvate dehydrogenase complex.,similarity:Belongs to the isocitrate and isopropylmalate dehydrogenases family.,subunit:Homodimer.,

Cellular Localization:

Mitochondrion .

Tissue Expression:

Colon,Heart,Kidney,Skin,

Research Areas:

>>Citrate cycle (TCA cycle) ;
>>Glutathione metabolism ;
>>Metabolic pathways ;
>>Carbon metabolism ;
>>2-Oxocarboxylic acid metabolism ;
>>Biosynthesis of amino acids ;
>>Peroxisome ;
>>Central carbon metabolism in cancer