Main Information

Target

Tubulin α

Host Species

Rabbit

Reactivity

Human, Mouse, Rat

Applications

WB, ELISA

MW

55kD (Observed)

Conjugate/Modification

Unmodified

Detailed Information

Recommended Dilution Ratio

WB 1:500-2000; ELISA 1:10000-20000

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Specificity

The antibody detects endogenous Tubulin α

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

Concentration

1 mg/ml

MW(Observed)

55kD

Modification

Unmodified

Clonality

Polyclonal

Isotype

IgG

Antigen&Target Information

Immunogen:

Synthetic peptide from human protein at AA range: 380-420

Specificity:

The antibody detects endogenous Tubulin α

Gene Name:

Tubulin alpha

Protein Name:

Tubulin alpha

Other Name:

Alpha-tubulin 1 antibody ALS22 antibody B ALPHA 1 antibody bA408E5.3 antibody H2 ALPHA antibody Hum a tub1 antibody Hum a tub2 antibody LIS3 antibody MGC171407 antibody MGC55332 antibody TBA4A_HUMAN antibody Testis-specific alpha-tubulin antibody TUBA1 antibody TUBA1A antibody tuba1l antibody Tuba4a antibody Tubulin alpha 1 chain antibody Tubulin alpha antibody Tubulin alpha-1 chain antibody tubulin alpha-1B chain antibody Tubulin alpha-4A chain antibody Tubulin H2-alpha antibody Tubulin, alpha 1 ;
testis specific ;
antibody tubulin, alpha 1, like antibody Tubulin, alpha 4a antibody Tubulin, alpha, testis-specific antibody Tubulin, alpha-1 antibody

Database Link:

Organism	Gene ID	SwissProt
Human	7846;10376;84790;113457;112714;7277;	Q71U36;P68363;Q9BQE3;Q13748;P68366;Q9NY65;

Background:

Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. This gene encodes alpha tubulin and is highly similar to the mouse and rat Tuba1 genes. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. This gene is one of three alpha-tubulin genes in a cluster on chromosome 12q.

Function:

Disease:Defects in TUBA1A are the cause of lissencephaly type 3 (LIS3) [MIM:611603]. LIS is characterized by a smooth brain surface due to the absence (agyria) or reduction (pachygyria) of surface convolutions. It is often associated with psychomotor retardation and seizures. LIS3 features include agyria or pachygyria or laminar heterotopia, severe mental retardation, motor delay, variable presence of seizures, and abnormalities of corpus callosum, hippocampus, cerebellar vermis and brainstem.,Function:Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.,PTM:Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively.,similarity:Belongs to the tubulin family.,subunit:Dimer of alpha and beta chains.,tissue specificity:Expressed at a high level in fetal brain.,

Cellular Localization:

Cytoplasm, cytoskeleton.

Tissue Expression:

Expressed at a high level in fetal brain.

Research Areas:

>>Phagosome ;
>>Apoptosis ;
>>Tight junction ;
>>Gap junction ;
>>Alzheimer disease ;
>>Parkinson disease ;
>>Amyotrophic lateral sclerosis ;
>>Huntington disease ;
>>Prion disease ;
>>Pathways of neurodegeneration - multiple diseases ;
>>Pathogenic Escherichia coli infection ;
>>Salmonella infection