Main Information

Target

AMPKα2

Host Species

Rabbit

Reactivity

Human, Mouse, Rat

Applications

WB

MW

62kD (Observed)

Conjugate/Modification

Unmodified

Detailed Information

Recommended Dilution Ratio

WB 1:1000-2000

Formulation

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Specificity

The antibody detects endogenous AMPKα2 protein.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

MW(Observed)

62kD

Modification

Unmodified

Clonality

Polyclonal

Isotype

IgG

Antigen&Target Information

Immunogen:

Recombinant Protein of AMPKα2

Specificity:

The antibody detects endogenous AMPKα2 protein.

Gene Name:

PRKAA2

Protein Name:

5'-AMP-activated protein kinase catalytic subunit alpha-2

Other Name:

PRKAA2 ;
AMPK ;
AMPK2 ;
5'-AMP-activated protein kinase catalytic subunit alpha-2 ;
AMPK subunit alpha-2 ;
Acetyl-CoA carboxylase kinase ;
ACACA kinase ;
Hydroxymethylglutaryl-CoA reductase kinase ;
HMGCR kinase

Database Link:

Organism	Gene ID	SwissProt
Human	5563;	P54646;
Mouse		Q8BRK8;
Rat		Q09137;

Background:

The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia. [provided by RefSeq, Jul 2008],

Function:

Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Binding of AMP results in allosteric activation, inducing phosphorylation on Thr-172 by STK11 in complex with STE20-related adapter-alpha (STRAD alpha) pseudo kinase and CAB39. Also activated by phosphorylation by CAMKK2 triggered by a rise in intracellular calcium ions, without detectable changes in the AMP/ATP ratio.,Function:Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Heterotrimer of a catalytic subunit, a beta and a gamma non-catalytic subunits.,

Cellular Localization:

Cytoplasm . Nucleus . In response to stress, recruited by p53/TP53 to specific promoters. .

Tissue Expression:

Heart,Skeletal muscle,

Research Areas:

>>FoxO signaling pathway ;
>>Autophagy - animal ;
>>mTOR signaling pathway ;
>>PI3K-Akt signaling pathway ;
>>AMPK signaling pathway ;
>>Longevity regulating pathway ;
>>Longevity regulating pathway - multiple species ;
>>Apelin signaling pathway ;
>>Tight junction ;
>>Circadian rhythm ;
>>Thermogenesis ;
>>Insulin signaling pathway ;
>>Adipocytokine signaling pathway ;
>>Oxytocin signaling pathway ;
>>Glucagon signaling pathway ;
>>Insulin resistance ;
>>Non-alcoholic fatty liver disease ;
>>Alcoholic liver disease ;
>>Hypertrophic cardiomyopathy ;
>>Fluid shear stress and atherosclerosis