Main Information

Target

GABA T-1

Host Species

Rabbit

Reactivity

Human, Rat, Mouse

Applications

WB, IHC, IF

MW

67kD (Observed)

Conjugate/Modification

Unmodified

Detailed Information

Recommended Dilution Ratio

WB 1:1000-2000; IHC 1:100-200; IF 1:50-200

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Specificity

GABA Transporter 1 protein(A230) detects endogenous levels of GABA Transporter 1

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

Concentration

1 mg/ml

MW(Observed)

67kD

Modification

Unmodified

Clonality

Polyclonal

Isotype

IgG

Antigen&Target Information

Immunogen:

Synthetic Peptide of GABA Transporter 1 AA range: 329-379

Specificity:

GABA Transporter 1 protein(A230) detects endogenous levels of GABA Transporter 1

Gene Name:

SLC6A1

Protein Name:

Sodium- and chloride-dependent GABA transporter 1 (GAT-1) (Solute carrier family 6 member 1)

Other Name:

SLC6A1 ;
GABATR ;
GABT1 ;
GAT1 ;
Sodium- and chloride-dependent GABA transporter 1 ;
GAT-1 ;
Solute carrier family 6 member 1

Database Link:

Organism	Gene ID	SwissProt
Human	6529;	P30531;
Mouse		P31648;
Rat		P23978;

Background:

The SLC6A1 gene encodes a gamma-aminobutyric acid (GABA) transporter, which removes GABA from the synaptic cleft (Hirunsatit et al., 2009 [PubMed 19077666]).[supplied by OMIM, Jul 2009],

Function:

Domain:The PDZ domain-binding motif is involved in the interaction with MPP5.,Function:Terminates the action of GABA by its high affinity sodium-dependent reuptake into presynaptic terminals.,miscellaneous:This protein is the target of psychomotor stimulants such as amphetamines or cocaine.,similarity:Belongs to the sodium:neurotransmitter symporter (SNF) family.,subcellular location:Localized at the plasma membrane and in a subset of intracellular vesicles. Localized at the presynaptic terminals of interneurons.,subunit:Interacts with MPP5.,

Cellular Localization:

Cell membrane; Multi-pass membrane protein. Membrane; Multi-pass membrane protein. Cell junction, synapse, presynapse . Localized at the plasma membrane and in a subset of intracellular vesicles. Localized at the presynaptic terminals of interneurons (By similarity). .

Tissue Expression:

Brain,

Research Areas:

>>Synaptic vesicle cycle ;
>>GABAergic synapse