Recommended Dilution Ratio
WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:40000; IF 1:50-200
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Specificity
The antibody detects endogenous Glutamate Receptor 1 protein
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage
-15°C to -25°C/1 year(Do not lower than -25°C)
Immunogen:
Synthetic Peptide of Glutamate Receptor 1 AA range: 773-823
Specificity:
The antibody detects endogenous Glutamate Receptor 1 protein
Protein Name:
Glutamate receptor 1 (GluR-1) (AMPA-selective glutamate receptor 1) (GluR-A) (GluR-K1) (Glutamate receptor ionotropic, AMPA 1) (GluA1)
Other Name:
Glutamate receptor 1 ;
GluR-1 ;
AMPA-selective glutamate receptor 1 ;
GluR-A ;
GluR-K1 ;
Glutamate receptor ionotropic, AMPA 1 ;
GluA1 ;
Background:
Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. This gene belongs to a family of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],
Function:
Function:Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.,miscellaneous:The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds AMPA (quisqualate) > glutamate > kainate.,PTM:Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.,similarity:Belongs to the glutamate-gated ion channel (TC 1.A.10) family.,subcellular location:Interaction with CACNG2 promotes cell surface expression.,subunit:Homotetramer or heterotetramer of pore-forming glutamate receptor subunits. Tetramers may be formed by the dimerization of dimers. Interacts with DLG1 via its C-terminus. Interacts with CACNG2, HIP1 and RASGRF2.,tissue specificity:Widely expressed in brain.,
Cellular Localization:
Cell membrane ; Multi-pass membrane protein . Endoplasmic reticulum membrane ; Multi-pass membrane protein . Cell junction, synapse, postsynaptic cell membrane ; Multi-pass membrane protein . Cell junction, synapse, postsynaptic density membrane ; Multi-pass membrane protein . Cell projection, dendrite . Cell projection, dendritic spine . Early endosome membrane ; Multi-pass membrane protein . Recycling endosome membrane ; Multi-pass membrane protein . Cell junction, synapse, presynapse . Cell junction, synapse . Interaction with CACNG2, CNIH2 and CNIH3 promotes cell surface expression. Colocalizes with PDLIM4 in early endosomes. Displays a somatodendritic localization and is excluded from axons in neurons (By similarity). Localized to cone photoreceptor pedicles (By similarity). .
Tissue Expression:
Widely expressed in brain.
Research Areas:
>>cAMP signaling pathway ;
>>Neuroactive ligand-receptor interaction ;
>>Circadian entrainment ;
>>Long-term potentiation ;
>>Retrograde endocannabinoid signaling ;
>>Glutamatergic synapse ;
>>Dopaminergic synapse ;
>>Long-term depression ;
>>Amyotrophic lateral sclerosis ;
>>Huntington disease ;
>>Spinocerebellar ataxia ;
>>Pathways of neurodegeneration - multiple diseases ;
>>Amphetamine addiction ;
>>Nicotine addiction
