Main Information

Target

WIPI2

Host Species

Rabbit

Reactivity

Human, Mouse, Rat

Applications

IHC, IF

MW

49kD (Observed)

Conjugate/Modification

Unmodified

Detailed Information

Recommended Dilution Ratio

IHC 1:50-300; IF 1:50-200

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Specificity

The antibody detects endogenous WIPI2 protein

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

Concentration

1 mg/ml

MW(Observed)

49kD

Modification

Unmodified

Clonality

Polyclonal

Isotype

IgG

Antigen&Target Information

Immunogen:

Recombinant Protein of WIPI2

Specificity:

The antibody detects endogenous WIPI2 protein

Gene Name:

WIPI2 CGI-50

Protein Name:

WD repeat domain phosphoinositide-interacting protein 2 (WIPI-2) (WIPI49-like protein 2)

Other Name:

WD repeat domain phosphoinositide-interacting protein 2 ;
WIPI-2 ;
WIPI49-like protein 2 ;

Database Link:

Organism	Gene ID	SwissProt
Human	26100;	Q9Y4P8;
Mouse		Q80W47;
Rat		Q6AY57;

Background:

WD repeat domain, phosphoinositide interacting 2(WIPI2) Homo sapiens WD40 repeat proteins are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins, such as WIPI2, have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids (Proikas-Cezanne et al., 2004 [PubMed 15602573]).[supplied by OMIM, Mar 2008],

Function:

similarity:Contains 3 WD repeats.,tissue specificity:Ubiquitously expressed. Highly expressed in heart, skeletal muscle and pancreas. Expression is down-regulated in pancreatic and in kidney tumors.,

Cellular Localization:

Preautophagosomal structure membrane ; Peripheral membrane protein ; Cytoplasmic side . Localizes to omegasomes membranes which are endoplasmic reticulum connected structures at the origin of preautophagosomal structures. Enriched at preautophagosomal structure membranes in response to PtdIns3P. .

Tissue Expression:

Ubiquitously expressed (at protein level). Highly expressed in heart, skeletal muscle and pancreas. Expression is down-regulated in pancreatic and in kidney tumors.

Research Areas:

>>Autophagy - other ;
>>Autophagy - animal ;
>>Alzheimer disease ;
>>Amyotrophic lateral sclerosis ;
>>Huntington disease ;
>>Spinocerebellar ataxia ;
>>Pathways of neurodegeneration - multiple diseases ;
>>Shigellosis