Main Information

Target

Galectin-3

Host Species

Mouse

Reactivity

Human, Mouse

Applications

WB, IHC, IF, ELISA

MW

26kD (Observed)

Conjugate/Modification

Unmodified

Detailed Information

Recommended Dilution Ratio

WB 1:2000-5000; IHC 1:100-200; IF 1:50-200

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Specificity

Galectin-3 protein detects endogenous levels of Galectin-3

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

Concentration

1 mg/ml

MW(Observed)

26kD

Modification

Unmodified

Clonality

Monoclonal

Clone Number

8D7

Antigen&Target Information

Immunogen:

Protein

Specificity:

Galectin-3 protein detects endogenous levels of Galectin-3

Gene Name:

LGALS3

Protein Name:

Galectin-3 (Gal-3) (35 kDa lectin) (Carbohydrate-binding protein 35) (CBP 35) (Galactose-specific lectin 3) (Galactoside-binding protein) (GALBP) (IgE-binding protein) (L-31) (Laminin-binding protein) (Lectin L-29) (Mac-2 antigen)

Other Name:

LGALS3 ;
MAC2 ;
Galectin-3 ;
Gal-3 ;
35 kDa lectin ;
Carbohydrate-binding protein 35 ;
CBP 35 ;
Galactose-specific lectin 3 ;
Galactoside-binding protein ;
GALBP ;
IgE-binding protein ;
L-31 ;
Laminin-binding protein ;
Lectin L-29 ;
Mac-2 antigen

Database Link:

Organism	Gene ID	SwissProt
Human	3958;	P17931;
Mouse		P16110;

Background:

This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. The protein exhibits antimicrobial activity against bacteria and fungi. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2014],

Function:

Function:Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis.,online information:Galectin-3,similarity:Contains 1 galectin domain.,subcellular location:Cytoplasmic in adenomas and carcinomas. May be secreted by a non-classical secretory pathway and associate with the cell surface.,subunit:Probably forms homo- or heterodimers. Interacts with DMBT1 (By similarity). Forms a complex with the ITGA3, ITGB1 and CSPG4. Interacts with LGALS3BP, LYPD3, CYHR1 and UACA.,tissue specificity:A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages.,

Cellular Localization:

Cytoplasm . Nucleus. Secreted . Secreted by a non-classical secretory pathway and associates with the cell surface. Can be secreted; the secretion is dependent on protein unfolding and facilitated by the cargo receptor TMED10; it results in protein translocation from the cytoplasm into the ERGIC (endoplasmic reticulum-Golgi intermediate compartment) followed by vesicle entry and secretion (PubMed:32272059). .

Tissue Expression:

A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages. Expressed in fetal membranes.