Main Information

Target

Phosphotyrosine

Host Species

Mouse

Reactivity

Species independent

Applications

WB, IHC, IF

Conjugate/Modification

Unmodified

Detailed Information

Recommended Dilution Ratio

WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:40000,; IF 1:50-200

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Specificity

The antibody detects endogenous Phosphotyrosine Monoclonal Antibody(2C1)

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

Concentration

1 mg/ml

Modification

Unmodified

Clonality

Monoclonal

Clone Number

2C1

Antigen&Target Information

Immunogen:

Purified Protein

Specificity:

The antibody detects endogenous Phosphotyrosine Monoclonal Antibody(2C1)

Background:

The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation. Its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.