产品简介：
pEcoli-6xHN-GFPuv is a control vector that encodes a GFPuv fusion protein containing an N-terminal 6xHN tag (composed of 6 repeating His-Asn subunits) and an enterokinase cleavage (EK) site. High-level, IPTG-inducible expression of the fusion is driven by the T7 lac hybrid promoter (PT7 lac), which consists of the strong T7 promoter and the lac operator. Basal expression of the fusion is repressed by the Lac repressor (lacI), which binds to the lac operator, preventing expression from the promoter in the absence of IPTG. High-level, IPTG-inducible expression of 6xHN-tagged GFPuv is driven by the T7 promoter in the presence of T7 RNA polymerase.

 

产品参数：

质粒类型：大肠杆菌表达载体
出品公司：Clontech

克隆方法：多克隆位点，限制性内切酶

启动子：T7 lac
载体大小：6346 bp
载体标签：6xHN tag（N-terminal）
载体抗性：Ampicillin

 

 

 

 

注意事项：
1、为了您的安全和健康，请穿实验服并戴一次性手套操作。本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗等其他用途。
2、使用甘油菌可不完全融解，在菌液表面蘸取少量涂布固体琼脂平板即可。完全融解后使用，菌体会逐渐沉淀至管底，请混匀后使用。随着冻融次数的增加，菌株的活力会逐渐下降。