Basic product information:

质粒简介：

pmCherry-N1 is a mammalian expression vector designed to express a protein of interestfused to the N-terminus of mCherry, a mutant fluorescent protein derived from thetetrameric Discosoma sp. red fluorescent protein, DsRed (1). The excitation and emission maxima of the native mCherry protein are 587 nm and 610 nm, respectively. Expression of fusion proteins that retain the fluorescent properties of the unmodified mCherry protein can be monitored by flow cytometry and their localization in vivo can be determined by fluorescence microscopy.

  

                                                   

产品参数：

出品公司：Clontech

启动子: CMV

复制子: pUC ori,f1 ori

终止子: SV40 poly(A) signal

质粒分类: 哺乳细胞，荧光蛋白报告载体

质粒大小: 4722bp

原核抗性: Kan

筛选标记: Neo

克隆菌株: DH5α

培养条件: 37℃，有氧 LB

表达宿主: 哺乳细胞

诱导方式: 无须诱导，瞬时表达

5'测序引物: CMV-F：CGCAAATGGGCGGTAGGCGTG

 

3'测序引物: mCherry-R:TTGGTCACCTTCAGCTTG

Other technical information

For more information, please download and refer to the product manual。