产品简介：

pTet-Off expresses the tet-responsive transcriptional activator (tTA) from the strong immediate early promoter of cytomegalovirus (PCMV). tTA is a fusion of amino acids 1–207 of the tet repressor (TetR) and the negatively charged C-terminal activation domain (130 amino acids) of the VP16 protein of herpes simplex virus. pTet-Off was originally described as pUHD15-1neo by Resnitzky et al. (1994) and was created by insertion of a neomycin resistance gene into pUHD15-1 (Gossen & Bujard, 1992). pTet-Off can be distinguished from pTet-On by digestion with Hind III.

产品参数：

质粒类型：四环素调控系统

出品公司：Clontech

启动子：CMV

克隆方法：多克隆位点，限制性内切酶

载体大小：7391 bp 

载体抗性：Ampicillin (氨苄青霉素)

筛选标记：Neomycin (新霉素)