pAAV-IRES-hrGFP 载体
载体类型:    腺相关病毒系统
表达水平:    高
启动子:    CMV
载体大小:    6.1 kb
3‘ 端测序引物:    Beta-globin-F：ATTCTGAGTCCAAGCTAGGC
5‘ 端测序引物:    hGH-PA-R: CCAGCTTGGTTCCCAATAGA
载体抗性:    Ampicillin (氨苄青霉素)

载体描述
The vector pAAV-IRES-hrGFP contains a dicistronic expression cassette in which the Renilla reniformis green fluorescent protein (hrGFP) gene is expressed as a second open reading frame downstream of the encephalomyocarditis virus (EMCV) internal ribosome entry site (IRES). The hrGFP gene serves as a useful marker for titering the virus and ascertaining the infection efficiency for the desired target cell type. Another unique feature of this vector is the FLAG sequence (3x) at the c-terminal of MCS. The foreign gene could be cloned in frame with the tag and the protein could be further purified or detected by anti-FLAG antibody.