载体介绍  ：

he vector pShuttle-CMV contains a multiple cloning site sandwiched between the CMV promoter and the SV40 polyadenylation signal and is suitable for insertion of a large cDNA (up to 6.6 kb). pShuttle contains only a multiple cloning site. This allows for the insertion of an entire expression cassette, including specialized promoters and termination signals (up to 7.5 kb). The regions indicated as arms are the stretches of sequence homology with pAdEasy-1 where the homologous recombination occurs. The R-ITR and L-ITR regions are short inverted terminal repeats (Left and Right) which have a role in replication of the viral DNA.

Vector pShuttle-CMV is used for expression of transgenes under a CMV promoter when no GFP tracer is desired.
Max insert size = 6.6kb with pAdEasy?1 and 9.3kb with pAdEasy?2.

产品参数:

出品公司:Stratagene

质粒类型：腺病毒系统

高拷贝/低拷贝：低拷贝

启动子：CMV

克隆方法：多克隆位点，限制性内切酶

载体大小：7469 bp

5' 测序引物及序列：pShuttle-CMV-F:GGTCTATATAAGCAGAGCTG

3' 测序引物及序列：pShuttle-CMV-R:GTGGTATGGCTGATTATGATCAG

载体抗性：Kanamycin (卡那霉素)