产品简介：

The pET-32a(+) is designed for cloning and high-level expression of peptide sequences fused with the 109aa Trx•Tag™ thioredoxin protein. Cloning sites are available for producing fusion proteins also containing cleavable His•Tag® and S•Tag™ sequences for detection and purification. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circle map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand. Therefore, single-stranded sequencing should be performed using the T7 terminator primer.

产品参数：

载体类型：原核表达载体

出品公司：Novagen

表达水平：高

克隆方法：多克隆位点，限制性内切酶

载体大小：5900 bp

启动子：T7

关键功能：IPTG诱导表达

原核抗性：Amp

检测标签：Trx•Tag，S•Tag，His•Tag

5' 测序引物：T7（5'-TAATACGACTCACTATAGGG-3'）

3' 测序引物：T7-ter（5'-GCTAGTTATTGCTCAGCGG-3'）

载体标签：thioredoxin (N端)；His (中间和C端)

载体抗性：Ampicillin (氨苄青霉素)

备注：pET-32a(+)载体用于表达可溶性的、具有活性功能的蛋白。pET-32a(+)载体具有N端Thrombin（凝血酶）酶切位点，N端肠激酶酶切位点。pET-32a(+)、pET-32b(+)及pET-32c(+)载体的差异主要是在于多克隆位点（MCS），其他地方完全一致。