pAc5.1/V5-His is a 5.4 kb expression vector designed for use with the Drosophila Constitutive Expression System available from Invitrogen. Upon transfection, the vector allows transient expression of your protein of interest in Drosophila cells. When cotransfected with the selection vector, pCoHygro or pCoBlast, included with the appropriate DES Constitutive Kit, pAc5.1/V5-His allows selection of stable cell lines exhibiting constitutive expression of the protein of interest. The Drosophila actin 5C (Ac5) promoter for high-level, constitutive expression of the gene of interest in S2 cells. C-terminal peptide containing the V5 epitope and polyhistidine (6xHis) tag for detection and purification of your protein of interest.