pTA-Luc载体

质粒类型:    信号通路报告载体

克隆方法:    多克隆位点，限制性内切酶

载体大小:    4871 bp  

载体抗性:    Ampicillin (氨苄青霉素)

出品公司： Clontech

备注:    uciferase reporter. For studying the transcriptional activity of enhancers.
 

载体描述
pTA-Luc is a member of the MercuryTM product line of signal transduction reporter vectors. This vector is designed for analyzing enhancer sequences by assaying for expression of the firefly luciferase (luc) gene from Photinus pyralis (1). In addition, pTA-Luc contains the minimal TA promoter, the TATA box from the herpes simplex virus thymidine kinase promoter (PTA). Located downstream of PTA is the luciferase reporter gene. pTA-Luc vector maintains constitutive levels of luciferase in transformed cells.

The vector backbone also contains a pUC origin of replication, and an ampicillin resistance gene for propagation and selection in E. coli.

载体应用
pTA-Luc is provided as a control for monitoring constitutive levels of luciferase activity in MercuryTM pathway profiling experiments. This vector is ideal for use as a negative control or for studying putative enhancers that are inserted upstream of the luciferase reporter gene. Luciferase is a highly sensitive enzymatic reporter that can be assayed by any standard luciferase-detection method, providing quantitative data on induction levels. pTA-Luc can be transfected into mammalian cells by any standard method. For selecting stable clones, cotransfect with a vector containing an antibiotic resistance gene, such as neomycin, hygromycin, or puromycin, and select resistant clones.