Fluorescence Resonance Energy Transfer(FRET) can be used to study protein-protein interactions in living cells. Protein interactions are critical formany processes inmammalian cells. Such interactions include the stable association of proteins within multi-subunit complexes and the transient association of regulatory proteins. Information about protein interactions in cells has previously come from either in vitro analyses using recombinant expressed proteins, or from yeast 2–hybrid studies. A limitation of this approach is that the protein interaction is studied in isolation, without regard to the many competing protein interactions that can occur within cells.
|pCLIP f -NK1R|