规格 | 目录价 |
促销价 | 库存 |
购买 |
---|---|---|---|---|
20 reactions | 1350.00 | 1350.00 | 0 |
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图片描述: | ||||
20 reactions |
产品简介:
The pIEx/Bac™ vectors are dual-purpose vectors that offer unique flexibility for cloning and expression of proteins in Spodoptera-derived insect cells. These vectors contain the Autographa californica nuclear polyhedrosis virus (AcNPV) enhancer/immediate early promoter combination (hr5/ie1) for plasmid-mediated and early baculovirus expression, and the AcNPV p10 very late promoter for robust baculovirus-mediated expression. All pIEx/Bac plasmids carry AcNPV-derived virus regions to mediate recombination at the polh locus. The pIEx/Bac-3 vector contains an N-terminal His•Tag® coding sequence with 10 histidines followed by an HRV 3C protease cleavage site and a multiple cloning site, with the option to include a C-terminal thrombin protease cleavage site and 8 aa Strep•Tag® II coding sequence. pIEx/Bac-3 is also available as a 3C/LIC vector. The 3C/LIC vector is prepared for rapid directional cloning of PCR-amplified DNA. Using specifically designed primers for amplification, inserts can be efficiently cloned without the need for restriction enzyme digestion or ligation.
产品参数:
质粒类型:昆虫细胞表达系统
出品公司:Novagen
启动子:IE1,p10
表达水平:高
克隆方法:多克隆位点,限制性内切酶
载体大小:6802 bp
载体标签:C-His,N-Strep
载体抗性:Ampicillin (氨苄青霉素)
备注:带有hr5增强子
产品名称 | 缩略图 |
---|---|
pFastBac1 | |
pFastBac1-Gus | |
pFastBacHT A | |
pFastBacHT B | |
pFastBacHT C | |
pFastBacHT-CAT | |
pFastBacDual | |
pBlueBacHis2 A | |
pBlueBacHis2 B | |
pBlueBacHis2 C | |
pVL1392 | |
pVL1393 | |
pDEST 10 | |
pIZT/V5-His | |
pMIB/V5-His B |
更多信息请下载和查阅产品说明书。